Journal: European Journal of Histochemistry : EJH

Article Title: Expressions of ZNF436, β-catenin, EGFR, and CMTM5 in breast cancer and their clinical significances

doi: 10.4081/ejh.2021.3173

Figure Lengend Snippet: Main experimental reagents.

Article Snippet: DAB colour-developing reagent , DA1010 , Solarbio , China.

Techniques:

Journal: Molecules

Article Title: Synthesis, Internalization and Visualization of N -(4-Carbomethoxy) Pyrrolidone Terminated PAMAM [G5:G3-TREN] Tecto(dendrimers) in Mammalian Cells †

doi: 10.3390/molecules25194406

Figure Lengend Snippet: Zeta potentials of dsDNA and PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer) (Structure (C)) solutions and their respective dsDNA: PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer) (Structure (C)) polyplexes formed at different DNA to dendrimer weight ratios. All data were measured at 25 °C. Error bars represent minimal and maximal values; boxes represent standard error of mean ( n = 4).

Article Snippet: All PAMAM dendrimer reagents (i.e., core reagent-[DAB:core]-dendri-{poly(amidoamine)-(NH 2 ) 128 }(PAMAM)(G5) and shell reagent-[DAB:core]-dendri-{poly(amidoamine)-(CO 2 Me) 32 } (PAMAM);(G2.5)) were provided by NanoSynthons, LLC, Mt.

Techniques:

Journal: Molecules

Article Title: Synthesis, Internalization and Visualization of N -(4-Carbomethoxy) Pyrrolidone Terminated PAMAM [G5:G3-TREN] Tecto(dendrimers) in Mammalian Cells †

doi: 10.3390/molecules25194406

Figure Lengend Snippet: Excitation and emission fluorescence spectra of 1 mg/mL PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer), Structure (C) in PBS.

Article Snippet: All PAMAM dendrimer reagents (i.e., core reagent-[DAB:core]-dendri-{poly(amidoamine)-(NH 2 ) 128 }(PAMAM)(G5) and shell reagent-[DAB:core]-dendri-{poly(amidoamine)-(CO 2 Me) 32 } (PAMAM);(G2.5)) were provided by NanoSynthons, LLC, Mt.

Techniques: Fluorescence

Journal: Molecules

Article Title: Synthesis, Internalization and Visualization of N -(4-Carbomethoxy) Pyrrolidone Terminated PAMAM [G5:G3-TREN] Tecto(dendrimers) in Mammalian Cells †

doi: 10.3390/molecules25194406

Figure Lengend Snippet: Fluorescence lifetimes (τ) and intensity fractions ( f ) or respective fluorescence decay curve components of PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer) (C) (pH 7.4). Values are shown as average ± s.e.m. ( n = 4).

Article Snippet: All PAMAM dendrimer reagents (i.e., core reagent-[DAB:core]-dendri-{poly(amidoamine)-(NH 2 ) 128 }(PAMAM)(G5) and shell reagent-[DAB:core]-dendri-{poly(amidoamine)-(CO 2 Me) 32 } (PAMAM);(G2.5)) were provided by NanoSynthons, LLC, Mt.

Techniques: Fluorescence

Journal: Molecules

Article Title: Synthesis, Internalization and Visualization of N -(4-Carbomethoxy) Pyrrolidone Terminated PAMAM [G5:G3-TREN] Tecto(dendrimers) in Mammalian Cells †

doi: 10.3390/molecules25194406

Figure Lengend Snippet: Cytotoxic effects of PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer) (C) and PAMAM (G5) dendrimer on HeLa and HMEC-1 cells. Cell viability was determined by the resazurin assay after 24 h treatment with dendrimers. Data are presented as percentage of viability of control (untreated) cells. Error bars represent the standard error ( n = 10).

Article Snippet: All PAMAM dendrimer reagents (i.e., core reagent-[DAB:core]-dendri-{poly(amidoamine)-(NH 2 ) 128 }(PAMAM)(G5) and shell reagent-[DAB:core]-dendri-{poly(amidoamine)-(CO 2 Me) 32 } (PAMAM);(G2.5)) were provided by NanoSynthons, LLC, Mt.

Techniques: Resazurin Assay, Control

Journal: Molecules

Article Title: Synthesis, Internalization and Visualization of N -(4-Carbomethoxy) Pyrrolidone Terminated PAMAM [G5:G3-TREN] Tecto(dendrimers) in Mammalian Cells †

doi: 10.3390/molecules25194406

Figure Lengend Snippet: Representative confocal images of untreated (control) and incubated for 24 h with PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer) (C) with HeLa cells. ( A ) . Cell nuclei were stained with RedDot1. Split images represent: a. cell nuclei, b. transmitted light image, c. blue fluorescence channel (intrinsic fluorescence of PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer) (C), d. merge of a., b. and c. ( B ). Cell nuclei were stained with RedDot1; plasma membranes were stained with NeuroDiO. Split images represent: a. plasma membrane, b. cell nuclei, c. blue fluorescence channel (intrinsic fluorescence of PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer) (C), d. merge of a., b. and c.

Article Snippet: All PAMAM dendrimer reagents (i.e., core reagent-[DAB:core]-dendri-{poly(amidoamine)-(NH 2 ) 128 }(PAMAM)(G5) and shell reagent-[DAB:core]-dendri-{poly(amidoamine)-(CO 2 Me) 32 } (PAMAM);(G2.5)) were provided by NanoSynthons, LLC, Mt.

Techniques: Control, Incubation, Staining, Fluorescence, Clinical Proteomics, Membrane

Journal: Molecules

Article Title: Synthesis, Internalization and Visualization of N -(4-Carbomethoxy) Pyrrolidone Terminated PAMAM [G5:G3-TREN] Tecto(dendrimers) in Mammalian Cells †

doi: 10.3390/molecules25194406

Figure Lengend Snippet: Representative confocal images of HeLa cells transfected using PAMAM G5 ( A ) and PAMAM [G5:G3-(TREN)]- N -(4-carbomethoxy) pyrrolidone terminated tecto(dendrimer) (Structure (C)) ( B ). Dendrimers were complexed at 8:1 N/P ratio with expression plasmid encoding TagGFP2, and cells were incubated with this complex for 24 h. Split images represent: a. TagGFP2 fluorescence (green channel), b. nontraditional intrinsic luminescence of the dendrimer (NTIL) (blue channel), c. transmitted light image, d. merge of a., b. and c.

Article Snippet: All PAMAM dendrimer reagents (i.e., core reagent-[DAB:core]-dendri-{poly(amidoamine)-(NH 2 ) 128 }(PAMAM)(G5) and shell reagent-[DAB:core]-dendri-{poly(amidoamine)-(CO 2 Me) 32 } (PAMAM);(G2.5)) were provided by NanoSynthons, LLC, Mt.

Techniques: Transfection, Expressing, Plasmid Preparation, Incubation, Fluorescence